Recombinant antibodies
You send the sequence. We send back purified, characterised antibody.
Full-length IgG, fragments and single domains, expressed in the system that suits the format. No immunisation, no hybridomas — this is recombinant production from a sequence you already have.
31working days
Sequence to purified IgG
50–300mg / L
Typical transient IgG yield
6formats
IgG, Fab, scFv, VHH, Fc fusion, bispecific
RUO
Research grade, not therapeutic
Formats
Six formats, each in the system that actually suits it.
One convention runs through the drawings below: a bar is a polypeptide chain, and the darker green is the variable region that does the binding.
Full-length IgG
CHOHuman IgG1, IgG2 and IgG4, plus mouse and rabbit backbones. Protein A capture, non-reduced purity check included.
Fab / F(ab′)₂
CHO or E. coliWhere the Fc gets in the way — structural work, tissue penetration studies, or avoiding Fc receptor binding.
scFv
E. coliFast and cheap. Good for screening panels of binders before committing to full IgG.
VHH / nanobody
E. coliSingle domain, expresses well in bacteria, our quickest route to a working binder.
Fc fusion
CHOReceptor ectodomains, traps and decoys, purified on Protein A like an antibody.
Bispecific
CHOKnob-into-hole and similar formats. Tell us the architecture — assembly and purity strategy depend on it.
Specification
Standard IgG project
The most common order: two sequences in, purified and characterised antibody out.
Research use only. Nothing we supply is intended for administration to humans or animals, or for use as a component of a diagnostic device.
| Input | Heavy and light chain variable sequences, or full constructs |
|---|---|
| Isotypes | Human · Mouse · Rabbit — human as IgG1, IgG2 or IgG4 |
| Host | Transient CHO (default) or HEK293 |
| Purification | Protein A capture, then size-exclusion polishing |
| Typical yield | 50–300 mg per litre of culture |
| Purity | Typically >95% monomer after polishing, assessed by size-exclusion |
| QC supplied | SDS-PAGE reduced and non-reduced, A280 concentration, size-exclusion profile |
| Turnaround | 31 working days from sequence |
| Format | Sterile-filtered, flash-frozen aliquots in PBS or your buffer |
| Add-ons | BLI affinity measurement · Western blot validation |
Pricing
Standard antibody project
from €4,200
recombinant IgG, 1 L transient CHO
Fragments and single domains expressed in E. coli are considerably cheaper — see the full price list. Bispecifics are quoted individually, because the architecture drives the work.
Questions
About recombinant antibodies
Do you raise antibodies, or only make them?
We make them. Send us sequences — from your own discovery campaign, a computational design, a published clone or a patent — and we express and purify them. We do not run immunisations or hybridoma work.
I have heavy and light chain sequences. What else do you need?
The isotype and species you want, the format, roughly how much material, and what you plan to do with it. That last one matters: material destined for a cell assay is handled differently from material going into a binding experiment.
Can you make an antibody described in a patent or a paper?
We can express a sequence you supply, and it is your responsibility to have the right to use it. We will not act as your freedom-to-operate assessment. Material is supplied for research use only in every case.
What about anti-idiotypic antibodies?
If you have the sequence, we express it like any other antibody. Discovery of a new anti-idiotypic binder against your drug is a different and much larger project — talk to us about it, but do not expect it on this timeline.
Do you measure binding?
Yes, by BLI — association and dissociation rates and an affinity constant, run against the antigen you supply or one we make for you. It is quoted as an add-on and takes about 5 working days, running alongside production rather than after it.
Will you supply antibodies for diagnostic kits?
Not as a regulated component. Everything we ship is research-grade and labelled research use only. If you are developing a diagnostic, you can use our material to explore and prototype, but the regulated supply chain is a different conversation with a different quality system behind it.
Have the sequences already?
Send the heavy and light chains, the isotype you want and roughly how much material. We will come back with a fixed price and a date.